Research GM Group
Concept: Observe, under an applied potential, the disruption of the flow of ions through single nanopores
Single molecules inside the nanopore are detected or studied by the modulation of the nanopore current
We pioneered the use of CyA and PlyAB nanopores for the analysis of proteins and double-stranded nucleic acids, and FraC nanopores for the sequencing of proteins.
Single-molecule identification of full-length proteins with single-amino-acid resolution using nanopores
Nanopore-based technologies show promise in single-molecule protein sequencing. By using an unfoldase and a nanopore with enhanced electroosmotic flow, here we show the continuous identification of generic proteins during single nanopore passes. This approach enables the recording of differences in charge and size from single amino acid substitutions compared to reference signals, paving the way for single-molecule protein sequencing and high-throughput proteomics.
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DOI: 10.64898/2026.01.08.698148
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